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The Smolka lab investigates signaling networks involved in genome maintenance and cell cycle control. We use mass spectrometry technologies, in combination with genetic and biochemical approaches, to elucidate the organization, dynamics and regulation of DNA damage signaling in yeast and mammals. Scroll down to learn more about our research projects.

WE ARE HIRING POSTDOCTORAL SCIENTISTS
Interested applicants: Please email CV to mbs266@cornell.edu

Recent Smolka Lab News:

  • March 2023: New review article in the Annual Reviews of Biochemistry: Checkpoint Responses to DNA Double-Strand Breaks. Great collaboration with the Haber Lab.
  • March 2023: Graduate student Ethan Sanford wins Top Poster Award at the Gordon Research Conference.
  • January 2023: Our new preprint shows that ATR inhibitors currently in clinical trials for anti-cancer treatment can be used as potent modulators of DNA end resection.
  • August 2023: Our new review article in Embo Journal: 梅林用$$R和V2玩联机延迟不理想,是不是用专门的加速器 ...:2021-5-27 · 川田五月 发表于 2021-5-8 10:09 加速器也不一定就理想,原理都是一样的 只是前期投资不一样而已 你买个一个月几百上千的线路,比任何加速器都 ...
  • 加速器比较好New work in collaboration with Jim Haber’s group on how to terminate the cellular response to DNA breaks: Mec1/ATR Autophosphorylation and Ddc2ATRIP Phosphorylation Regulates DNA Damage Checkpoint Signaling.
  • July 2023: We have ~doubled the phosphoproteome of budding yeast: In-depth and 3-Dimensional Exploration of the Budding Yeast Phosphoproteome.
  • May 2023: Congratulations to graduate student Jennie Sims for receiving the prestigious Mann Award.
  • May 2023: Graduate student Jumana Badar joins the lab. Welcome!
  • May 2023: Two new papers in collaboration with the Baskin and Koren labs: Cell Reports & Genes.
  • July 2018: Chronicle features our new paper: Study reveals how promising cancer drug works for best use
  • June 2018: New paper: Smolka lab reports a new role for a key anti-cancer target.
  • 十大加速器排名 Our research was featured on the Cornell Research Website
  • June 2018: Graduate students Mike Lanz and Dongsung Kim give talks at the Buffalo DNA Replication and Repair Symposium
  • May 2018: Graduate student Shannon Marshall joins the lab. Welcome!

Research Focus

The integrity of our genome is especially at risk while it is being replicated. During DNA replication, the DNA must be “unzipped”, giving rise to structures known as replication forks. While traversing the genome, replication forks often encounter obstacles to their progression, including DNA lesions, hard-to-replicate sequences, transcription intermediates, or protein-DNA complexes. These encounters are potential sources of DNA breaks, chromosomal rearrangements and aneuploidy, all of which are hallmarks and drivers of cancer. Proper control of replication fork progression is therefore essential for genome integrity and cancer avoidance. Paradoxically, numerous anti-cancer agents such as topoisomerase inhibitors, DNA crosslinkers and DNA alkylators, kill cancer cells by impairing the regulated progression of the replication machinery and inducing replication stress. Our laboratory studies how cells sense, signal and prevent DNA replication stress to ensure faithful genome replication. We seek a deep mechanistic and integrated understanding of the replicative stress response and its implications for tumorigenesis and improved cancer treatment.加速器比较好

NBA2K 17需要用加速器么?用什么加速器比较好 - Sohu:2021-9-10 · 那么玩NBA2K 17用什么加速器比较好 呢?虽然现在市场上加速器品牌繁多,但是真正起到加速作用的却不多。 玲珑玲珑是国内唯一一家使用独享高速网络的加速器厂商,保证玩家全天低延迟不丢包的游戏,让玩家愉快的玩耍。绑定手机号可伍获得 .... We are investigating how the actions of kinases are translated into a coordinated cellular response that ensures proper completion of genome replication. As shown in the figure below, our recent work using budding yeast as a model organism has uncovered how phosphorylation events mediated by CDK and checkpoint kinases form a “code” of combinatorial protein interactions that lead to distinct functional outputs, therefore allowing timely coordination of DNA replication, cell cycle and DNA repair (1-3). Central to this combinatorial mode of regulation is the multi-BRCT domain scaffold Dpb11, capable of “reading” a range of phosphorylation events and physically coupling distinct protein complexes. We are currently investigating how TOPBP1, the human ortholog of Dpb11, coordinates DNA damage responses via a similar phosphorylation-mediated code.

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Phosphoproteomics. Reversible protein phosphorylation is widely used by cells as a signaling mechanism. Understanding the molecular basis of kinase action and function requires knowledge of the kinase substrates, as well as comprehensive characterization of the dynamics and role of the phosphorylation events. Because many kinases are active in a cell and thousands of proteins are phosphorylated, the study of phosphorylation-mediated signaling pathways is challenging and powerful technologies are needed. We have developed and applied quantitative mass spectrometry technologies for the phosphorylation analysis of protein complexes and for global screens of in vivo kinase substrates (4). We are now expanding the use of these technologies to quantitatively characterize signaling dynamics and regulation at a proteome-wide scale.

kinase_prof

 

1. Cussiol, J.R., 十大加速器排名. EMBO J 34, 1704-17 (2015).

2. Ohouo, P.Y. et al. 加速器比较好 493, 120-4 (2013).

3. Ohouo, P.Y. et al. Mol Cell 39, 300-6 (2010).

4. Bastos-de-Oliveira, F., Kim, D.  et al. Mol Cell 57, 1-9 (2015).

 

We are grateful for the funding provided by:

十大加速器排名

NIGMS

 

 

 

 

 

十大加速器排名

  • November 2017: Our recent NIH R01 award “Cellular Responses to DNA Replication Stress” was featured by Cornell Research: http://research.cornell.edu/research/dna-damage-and-repair-implications-tumor-formation
  • November 2017: Labsgiving party:  
  • October 2017: Our methods paper about quantitative analysis of kinase action is published: http://www.ncbi.nlm.nih.gov/pubmed/29043653
  • October 2017: Another awesome Weill Institute Symposium:  http://wicmb.cornell.edu/news/symposium
  • August 2017: Summer Lab Barbecue: 十大加速器排名
  • 十大加速器排名BMCB student Ethan Sanford joins the lab. Welcome!

 

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十大加速器排名

《生死相依》上线steamdeadside好用加速器推荐 – raybet雷 ...:同时本作对于电脑的最低配置也比较伌人满,i3处理器+4G内存即可跑起来,安装容量则需要20GB。 目前国内已经确定《生死相依》这款游戏的线上联机功能需要加速器支持,不过因为游戏刚刚上线热度比预想的也要低一些,所伍支持的加速器还比较少。

Marcus B. Smolka, Ph.D
Weill Institute for Cell and Molecular Biology
Department of Molecular Biology and Genetics
Cornell University
339 Weill Hall
Ithaca, NY 14853-7202

mbs266@cornell.edu

(607)255-0274 (Office)
(607)255-0174 (Lab)

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Annual Review of Biochemistry (2023) - Checkpoint Responses to DNA Double-Strand Breaks. David Waterman, James Haber, Marcus Smolka.

十大加速器排名 (2023) - 打游戏老是延迟掉帧,鲁大师游戏加速器能改善吗?_中华网:2 天前 · 花钱买加速器结果最新游戏不支持加速、加速效果不理想依然卡顿掉线的现象也层出不穷。 今天小编给大家推荐的这款游戏加速器,不仅有强大的技术后台支撑,加速效果也比较好,就是鲁大师电竞加速器。 Mike Lanz, Diego Dibitetto, Marcus Smolka.

Preprint: BioRxiv (2023) - In-depth and 3-Dimensional Exploration of the Budding Yeast Phosphoproteome. Mike Lanz, Kumar Yugandhar, Shagun Gupta, Ethan Sanford, Vitor Faça, Stephanie Vega, Aaron Joiner, Chris Fromme, Haiyuan Yu, Marcus Smolka.

Genes & Development (2018) - Separable roles for Mec1/ATR in genome maintenance, DNA replication, and checkpoint signaling. Mike Lanz, Susannah Oberly, Etan Sanford,..., Marcus Smolka.

Nucleic Acids Research (2018) - ATR-mediated proteome remodeling is a major determinant of homologous recombination capacity in cancer cells. Dongsung Kim, Yi Liu, Susannah Oberly, Raimundo Freire and Marcus B Smolka.

J Cell Bio (2017) - TOPBP1/Dpb11 plays a conserved role in homologous recombination DNA repair through the coordinated recruitment of 53BP1/Rad9. Yi Liu, José Renato Cussiol, Jennie Rae Sims, …, Marcus Bustamante Smolka.
Commentary by the Gasser Lab: A game of musical chairs: Pro- and anti-resection factors compete for TOPBP1 binding after DNA damage.

J Cell Biol (2016) - TOPBP1 takes RADical command in recombinational DNA repair. Yi Liu and Marcus Smolka.

Mol Cell (2015) - Phosphoproteomics Reveals Distinct Modes of Mec1/ATR Signaling during DNA Replication. Francisco Bastos de Oliveira, Dongsung Kim, José Renato Cussiol, ... Marcus Smolka.

EMBO Journal (2015) - 英雄联盟手游加速器哪个好 lol手游加速器推荐 _3DM手游:2021-5-29 · 那么英雄联盟手游加速器选哪个比较好呢? 对于Lol英雄联盟手游的加速器,小编比较推荐奇游手游加速器,这款加速器提供了游戏下载-账号注册-加速登录-游戏新版本更新-人工客服咨询解决问题等一套服务,能够在这一款加速器里解决玩英雄联盟手游时几乎所有能遇到的问题。. José R Cussiol, Carolyn M Jablonowski, Askar Yimit, Grant W Brown, Marcus B Smolka

Nature (2013) - DNA Repair Scaffolds Dampen Checkpoint Signaling by Counteracting the Rad9 Adaptor. Patrice Ohouo, Yi Liu, Francisco M Bastos de Oliveira and Chu Jian "Frank" Ma and Marcus Smolka.

Cell Cycle (2012) - The many roads to checkpoint activation. Patrice Ohouo and Marcus Smolka.

加速器比较好 (2012) - The checkpoint transcriptional response: Make sure to turn it off once you are satisfied. Marcus Smolka, Francisco Bastos de Oliveira, Michael Harris, and Robertus A M de Bruin.

EMBO Journal (2012) - Linking DNA replication checkpoint to MBF cell-cycle transcription reveals a distinct class of G1/S genes. Francisco Bastos de Oliveira, Michael Harris, Pijus Brazauskas, Robertus A M de Bruin and Marcus Smolka.

Cell (2011) - TORC1 regulates endocytosis via Npr1-mediated phosphoinhibition of a ubiquitin ligase adaptor. JA MacGurn, PC Hsu, Marcus Smolka and Scott Emr. 147(5):1104-17.

Cell Cycle (2011) - A touching moment for Smc5/6: from ssDNA binding to repair. Patrice Ohouo, Marcus Smolka, (April 2011) (comment on Roy MA, et al. Cell Cycle).

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